Lab Report On Photosynthesis

The effect of different colo florid escapeds on the treasure of Photosynthesis Objective To risk aside which color of agility provides the shell consequences for the ware of group O/ the dictate of photosynthesisBackgroundIn photosynthesis, there atomic number 18 two main parts, including aerial dependent and shadowy-independent reactions. Plants use the energy from light for producing sugar, which is existence converted into ATP by cellular respiration. They also are the only organisms that produce type O on with glucose and fructose chains within the light-independent phases of photosynthesis. This turn takes place in the chloroplasts of im coiffes, which take on chlorophyll.Chlorophyll is essential for photosynthesis, since it absorbs the suns light. thou plants then use this light to combine CO and HO for making sugars and type O. after this process atomic number 8 is universe released though pores c completelyed stomata. The over every(prenominal) reaction w ashbowl be expressed as follows 6HO + 6CO CHO + 6O1There are four variables that affect the enume consecrate of photosynthesis light intensity, temperature, CO and body of wet.2 However, is it possible to deal in the strain of light, too, which I am going to investigate in this lab. Since the photosynthesis takes place in chloroplasts that has chlorophyll, the rate of photosynthesis can be affected by different colours of light, since the absorption of light changes within different colours. look into QuestionWhat colour of light is most effective on the rate of photosynthesis? Five different one-sided transparencies (red, green, yellow, aristocratical, purple) are macrocosmness used for ever-changing the lights colour. Within 5 minutes for each angiotensin-converting enzyme of them, they leave alone be used in order to see oxygen bubbles rising from a used water plant. The produced oxygen (in the form of bubbles) indicates the rate of photosynthesis and is being measur ed by counting bubbles. The highest amount of oxygen bubbles being produced per minute is equivalent to the highest rate of photosynthesis, and the colour providing the best circumstances for the exertion of photosynthesis. HypothesisSince Plants absorb green light, I expect the rate of photosynthesis to be the highest when red light is being used. This is due to the colourspectrum, where one can say that red is the opposite colour to green. Therefore, I expect red light to provide the most energy for the plant absorbing it. variables Variable Type Method Light intensity Controlled At the most acuate level possible (770 lux) and a distance of 50cm from the aquarium. Temperature of water Controlled At direction temperature adding colder water when temperature rises. pH level Controlled All solution has a pH of 7. Plant type and colour Controlled Leaves of the akin species green Coloured light IndependentChanging colours with coloured slides (yellow, blue, red, green, purple) in f ront of the light source. The rate of photosynthesisDependent Changed by the colour of light, collected with stop watch and counted by produced bubbles.Material 2000 cm of water (300 for ein truth colour + water for controlling variables) One flask/ glass cup One sample tube One displace 2-3 healthy water plants One thermometer Spotlight Five coloured transparencies (blue, red, yellow, green, purple) stop watch One lux meterProcedureBefore starting the experiment, the stain has to be fit(p) at point on the table, 20 cm away from where the plant is to be decorate. Then the lux meter is to be taken for cypher the intensity of the spotlight onto the plant. Following this, the colour transparencies will be set in front of the light and the resulting light intensity will be observe by placing the lux meter on the place where the plant is to be set. For each run, under-water cut leaves from the water plant are vomit up carefully in the funnel, which small open end is practice i nto the test tube. This all is to be done in the flask filled with the water provided for each run and is to be placed at the distance of 20 cm from the spotlight. 3 The water temperature is to be measured and to be unbroken constant at all while (+/- 1C).The first colour transparency is then to be placed in front of the spotlight and the oxygen bubbles rising from the plants leaves are to be counted. After 1 minute, the first run of the first colour is completed and undermentioned 4 runs have to be concluded the same way. In end the water temperature rises, cold water has to be added. The experiment then has to be repeated 4 more times with the different coloured transparencies in the same way. (A procedure repeating each run 5 times, would be the best for minimizing errors that could occur, due to the 55 rule.) The collected data is noted on paper and will be processed to determine the rate of photosynthesis at every colour.Data collection and raw processing The experiment w as carried out with the following order of colours Run 1-5 Blue Run 6-10 potassium Run 11-15 Yellow Run 16-20 Red Run 21-25 Purple affectionate Data Qualitative Observations Run 1-5 A high number of bubbles arise up Run 6-10 Almost no bubbles were being produced Run 11-15 barely a few bubbles rose up Run 16-20 A very large number of bubbles were being produced Run 21-25 Not too umpteen bubbles were being produced by the plant * Until the first bubbles rose up, about time has had passed already the observations are only being compared with the data processed by the plant being used in this experiment, not with those from others.Quantitative Data Run snatch of Oxygen bubbles being produced with Blue light Number of Oxygen bubbles being produced with Green light Number of Oxygen bubbles being produced with Yellow light Number of Oxygen bubbles being produced with Red light Number of Oxygen bubbles being produced with Purple light Run 1(after 90 sec.)4 2 0 0 3 2 Run 2 (after 180 s ec.) 5 11 1 4 13 4 Run 3 (after 270 sec.) 12 2 4 15 6 Run 4 (after 360 sec.) 12 1 2 14 5 Run 5 (after 450 sec.) 13 1 3 15 5For a better overview the diagram on a lower floor represents the quantitative data provided.Processed DataThe collected data has to be processed. This will be done by calculating the mean values for all runs of each of the colours by using Excel. The results will be put in a table, as well as in a diagram for a better overview. Also, for finding out whether the collected data is reliable or not and how different the results are to the means, the standard leaving for all runs of the colours have to be calculated using the same method.Bule Green Yellow Red Pruple Mean 10 1 2.6 12 4.4 Stand. Dev. 0.82 0.5 0.96 0.96 0.82 Table providing the processed dataDiagram providing the processed data for a better overviewConclusionAfter all data has been collected and processed, one can say that the stated possible action from the beginning of the lab report, which says th at red is the colour that provides the best circumstances for the yield of oxygen, is being supported by the observations and calculations made. The qualitative observations showed clearly, that the plant produced most oxygen under red light. The calculations of the means demonstrated that even though blue and red light had similar effects on the plant, the red light was most effective. Further calculations of the standard deviation showed that the data is not extremely spread, standing for valuable data that has been collected.Therefore it is to be concluded that the colour providing best light for the production of oxygen and therefore for the rate of photosynthesis is red, followed by blue. Furthermore, the data collected in this experiment is supported by sources and expectations from the IB Biology platform (and others), which states conclusions about the colour spectrum and the effect on plants absorbing the different wavelengths of coloured lights. However, it is to be note d that the data being collected points out some significant differences, as well as similar numbers of oxygen being produced.This is probably due to the small protection of the light overture from the other sides onto the plant, which was not shielded and completely darken except for the light coming from the spotlight. It is therefore more likely that the data collected was being influenced by the outside light, since there is a significant difference in the number of bubbles from the first run, in which few further light was influencing the production of oxygen. For improving the results, one could prepare the experiment in a darkened room, using more volt for the coloured lights.Another problem can be included with pointing onto the water, which, for better results und anda higher rate of photosynthesis, should be changed to tonic water, or at least, should provide a warm temperature and not as cold as the water was within Run No. 1. scour though there were these minor problem s and possible improvements to this experiment, it worked out quite well, since the results being portrayed fit to those that have been published before.